Table 1 Summary of transgenic clones generated in this study

From: Genetic and epigenetic features direct differential efficiency of Xist-mediated silencing at X-chromosomal and autosomal locations

Clone

Category

Chr.

Allele

Karyotype

5′–3′ Integration sites (mm10)

Additional information

87

Tg-E

X

Cast

40,XX

chrX:103,460,373-103,483,217

Xist promoter 1 kb del (Cast)

68

Tg-E

X

Cast

40,XX

chrX:103,460,373-103,483,217

Xist promoter 1 kb del (Cast)

64

Tg-E

X

Cast

40,XX

chrX:103,460,373-103,483,217

Xist promoter 1 kb del (Cast)

77

Tg-E

X

Cast

40,XX

chrX:103,460,373-103,483,217

Xist promoter 1 kb del (Cast)

267

Tg-X:8

X:8

129

40,XX,t(X;8)

chr8:110,645,220-110,329,507

Tsix-Stop allele (129)

339

Tg-X:8

X:8

129

40,XX,t(X;8)

ND

–

203b

Tg-X:8

X:8

129

40,XX,t(X;8)

chr8:122,340,941-122,343,259

–

86

Tg-X

X

Cast

40,XX,t(X;8)

chrX:134,402,074a-134,559,764

–

190

Tg-X

X

Cast

40,XX,t(X;8)

chrX:169,976,922a-170,005,736a

–

85

Tg-X

X

Cast

40,XX,t(X;8)

~chrX telomere

–

109

Tg-X

X

Cast

40,XX,t(X;8)

chrX:103,483,223a-103,484,258a

–

251

Tg-12

12

ND

41,XX,dup12

ND

–

292

Tg-12

12

Cast

41,XX,dup12

chr12:89,465,956a-89,155,600

–

228

Tg-12

12

Cast

41,XX,dup12

chr12:109,077,348-109,113,528

–

160

Tg-12

12

Cast

41,XX,dup12

~chr12 centromere

Tsix-Stop allele (129)

55

Tg-12

12

129

41,XX,dup12

chr12:104,543,748a-104,556,010

–

273

Tg-12

12

Cast

41,XX,dup12

chr12:98,483,300a-98,489,700

–

  1. TLA targeted locus amplification
  2. aDue to the features of the integration locus and/or the Xist transgene sequence, the initial TLA experiment did not yield the exact integration site, but did allow to make the approximation indicated