Fig. 2 | Nature Communications

Fig. 2

From: Fas cell surface death receptor controls hepatic lipid metabolism by regulating mitochondrial function

Fig. 2

Adenovirus-mediated hepatic overexpression of Fas induces hepatic steatosis and hepatic insulin resistance. a Protein levels of Fas in total liver lysate of mice injected with adenoviruses expressing either Fas (Ad-Fas) or a control vector (Ad-lacZ). b Body weight of mice 12 days after adenovirus injection, n = 7. c Liver TG (n = 5), ceramide, and DAG (Ad-LacZ, n = 6; Ad-Fas, n = 7) levels are shown. d Intraperitoneal glucose tolerance test (n = 7), e glucose infusion rate (GIR), and endogenous glucose production (EGP) during hyperinsulinemic-euglycemic clamps (n = 4) in mice 12 days after injection of Ad-lacZ or Ad-Fas are depicted. f Representative western blots of total liver lysate harvested from mice 15–16 days after injection of Ad-lacZ or Ad-Fas. Values are expressed as mean±s.e.m.; *p < 0.05, **p < 0.01, and ****p < 0.0001. Statistical tests used: t-test for (b, c (TG, ceramide), e); Mann–Whitney for (c (DAG)); ANOVA for (d)

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