Fig. 4
Functionality and protection by DMAb-αPcrV and DMAb-BiSPA in mice. a BALB/c mice were administered 300 µg of DMAb-αPcrV, DMAb-BiSPA, or MEDI3902 IgG (2 mg kg−1). n = 5 mice/group. Two animals from the DMAb-BiSPA were below the limit of detection of the anti-cytotoxic activity assay. Antibody levels are representative of DMAb in serum on the day of challenge. b In vivo protection in BALB/c mice following administration of control DMAb-DVSF3 (black open circles), DMAb-αPcrV (red circle), DMAb-BiSPA (green circle) or on day 5 or purified MEDI3902 mAb (purple circle) on day 1 before lethal challenge (data represented is from two independent experiments, n = 8/group/experiment, total n = 16). c Protection with different doses of DMAb-BiSPA: 100 µg (purple circle), 200 µg (green circle), 300 µg (red circle), or DMAb-DVSF3 (control). n = 8 mice/group. d Serum DMAb concentrations with different doses of DMAb-BiSPA. n = 8 mice/group
