Fig. 5
From: The autophagy initiator ULK1 sensitizes AMPK to allosteric drugs
ULK1 phosphorylation of Ser108 is specific for the AMPK β1 isoform. a Immunoblots for β1-pSer108, β2-pSer108, and α-pThr172 in bacterial-expressed KI-α1β1γ1 (left) or KI-α1β2γ1 (right) phosphorylated with ULK1 or CaMKK2 for 30 min. Controls: CaMKK2-treated WT α1β1γ1 (left) or α1β2γ1 (right). b Immunoblots for β-pSer108 in KI-α1β1γ1 (left) or KI-α1β2γ1 (right) purified from HEK293T cells stimulated with 1 mM H2O2 for 45 min. Controls: p-β1 and p-β2, CaMKK2-treated WT α1β1γ1 and α1β2γ1, respectively. In both panels n = 3, representative immunoblots shown
