Fig. 1 | Nature Communications

Fig. 1

From: Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons

Fig. 1

SMX setup, features and overview of results. The schematic of the SMX experimental setup at the Swiss Light Source shows the high-viscosity injector set to extrude a 50 µm diameter stream of crystal laden LCP (or other viscous carrier) vertically through the interaction region with the X-ray beam and into a catcher for safe and clean disposal of material. The micro-focused beam is centered on the extruded medium ~50 µm below the nozzle. The jet speed determines exposure time and is calculated from the nozzle diameter and sample flow rate (which is calculated from the pump flow rate). The extrusion is controlled with a helium sheath, which can be adjusted with the pressure regulator to maximize jet stability. A typical beam size in our experiments is 20 µm horizontal and 5 µm vertical with respect to the extruded medium. This size allows for a large interaction volume and maximum flux. Data are sent to the beamline data collection servers where real-time peak finding is fed back to the user

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