Fig. 4

Ribosome aggregation in cells lacking Ssb is due to reduced Sch9 activity. a, b Aggregation of ribosomal proteins in cells lacking Ssb and/or Sch9. Insoluble material was prepared as described in Methods section. The indicated fractions of total cell extract (total) and insoluble material (insol) were separated on Tris-Tricine gels and were subsequently analyzed via Coomassie staining (a) or immunoblotting (b) employing the antibodies indicated. c Hyperactive Sch9-2D3E partially rescues ribosome aggregation in cells lacking Ssb. The experiment was performed and analyzed as described in a, b. d Hyper-active Sch9-2D3E partially rescues Maf1 phosphorylation in cells lacking Ssb. Protein extracts from the indicated strains were analyzed as described in Fig. 3c. e Hyper-active Sch9-2D3E does not complement for the general growth defects of cells lacking Ssb. Logarithmically growing cells from the strains indicated were serially diluted, spotted on YPD plates, and were incubated at the indicated temperatures