Fig. 3
From: Understanding CRY2 interactions for optical control of intracellular signaling
Negative charges at the C-terminus of CRY2 inhibit CRY2 homo-oligomerization. COS7 cells were co-transfected with CIB1-GFP-Sec61 and each mCh-tagged CRY2, respectively. Blue light of 200-ms duration was delivered at 2-s intervals for 20 s (a–c) or one 100-ms pulse of blue light was delivered (d). a Increasing numbers of negative charges were introduced to the C-terminus of CRY2(1–488). b After recruitment to the ER membrane via CRY2–CIB1 hetero-dimerization, CRY2 derivatives with more C-terminal negative charges formed fewer clusters as compared to CRY2wt. c The cluster mass of each CRY2 mutant on ER membrane was separately quantified and then normalized to that of CRY2wt. Results are presented as means ± s.e.m. (n = 13, 12, 15, 14, 16, 12, 11, 13, 11, 15, 12) and analyzed using one-way ANOVA with Dunnett’s post hoc test. (*P < 0.05, ****P = 0.0001). d CRY2high, CRY2wt and CRY2low formed different amount of clusters on plasma membrane at different CRY2 concentrations. (CRY2high n = 48, CRY2wt n = 48, CRY2low n = 49) Scale bars, 5 µm
