Fig. 1
From: Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation
A subpopulation of MNTB OLs can fire APs. a Excitable OLs in the MNTB were subjected to whole-cell patch-clamp recording and were filled with Alexa 568 (red) during recording for post-recording immunostaining. Differential interference contrast (DIC) image of an excitable OL, which was loaded with Alexa 568 (red). Yellow arrows indicate MNTB principal neurons. Scale bar, 20 μm b A single AP was generated by a brief current injection (500 pA for 1.8 ms, left) in an excitable OL at P11. APs were evoked by step-current injections from −40 to 140 pA (300 ms, right) in the same cells. Inset, expanded time scale of area within the dashed box. c Upper Excitable OLs were immunostained for O1 (OL marker; green) and NeuN (neuronal marker; blue). A representative excitable OL displayed APs (inset) and expressed O1 (yellow arrow) but not NeuN (white arrow). Lower Excitable OLs were immunostained for GFAP (astrocyte marker; green) and NeuN (blue). A representative OL expressed neither GFAP nor NeuN (white arrow). Scale bar, 10 μm
