Fig. 2 | Nature Communications

Fig. 2

From: Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation

Fig. 2

Excitable OLs belong to the pre-myelinating OL stage. a Post-recording immunostaining of Alexa 568-filled excitable OLs (red) for CNPase (blue) and DM20-PLP (green, upper), NG2 (green, middle), or MBP (green, lower) in the MNTB (P9−P14). b Confocal image of CNPase-GFP+ cells in the MNTB that were infected by adenovirus encoding pAV.ExSi-CNPase promoter-eGFP. c CNPase-GFP+ pre-OLs in the MNTB. A CNPase-GFP+ pre-OL was filled with Alexa 568 during whole-cell recording, as demonstrated in differential interference contrast (DIC) and fluorescence images. Dashed lines indicate the patch pipette. The CNPase-GFP+ pre-OL fired APs in response to step-current injections of 80 and 120 pA (300 ms). Scale bar, 10 μm

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