Fig. 3
From: Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation
Morphology and physiological properties of excitable vs. non-excitable pre-OLs and mature OLs. a–c Confocal images of excitable pre-OLs a, non-excitable pre-OLs b, and mature OLs c, which were filled with Alexa 568 during whole-cell recordings. Scale bar, 10 μm. d–f Representative traces of Nav-mediated currents generated by voltage steps (from –100 to 40 mV for d and e, from –110 to 30 mV for f) in excitable pre-OLs, non-excitable pre-OLs and mature OLs. Gray arrows indicate both peak outward currents (Ipeak) and steady-state outward currents (Ipeak), and the red arrow indicates inward currents. Inset, expanded time scale of inward currents from excitable pre-OLs (scale; 2 ms and 400 pA, d). g–i Current–voltage (I–V) relationship for Ipeak (black), Isteady (gray), and Iinward (red) from excitable pre-OLs g. I-V relationship for Ipeak (black) and Isteady (gray) from non-excitable pre-OLs h and mature OLs i. Data represent the mean ± s.e.m. j Excitable pre-OLs fired APs in response to current injections (>50 pA, 300 ms) in current-clamp mode. k, l Recordings of membrane potential from a non-excitable pre-OL k and a mature OL l in response to current injections (300 ms, from –80 to 200 pA for k, and –50 to 400 pA for l). Dashed lines indicate the resting membrane potential
