Fig. 7
From: Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation
The population of excitable pre-OLs increases during early postnatal development in the MNTB. a Immunostaining for Nav1.2 channels (green), CNPase (blue) and NG2 (red) as well as DAPI staining (magenta) in the MNTB at P5 and P31. Yellow arrows indicate CNPase+/Nav1.2+ pre-OLs, and white arrows indicate NG2+ cells. Scale bar, 20 μm. b Total number of NG2+/CNPase– cells (gray), CNPase+ cells (black), and Nav1.2+/CNPase+ cells (excitable pre-OLs, red) from three slices (from four different brains) each at P5, P7, P9, P11, P13, P31, and P62). c Voltage-activated K+ and Na+ currents in response to step depolarization (−80 to 40 mV) in an excitable pre-OL (left) at P20. The same pre-OL exhibited APs in response to current injections (60–140 pA). Post-recording immunostaining revealed that the same cell (loaded with Alexa 568, red, arrow) expressed CNPase (blue) and Nav1.2 (green). Scale bar, 10 μm
