Fig. 8
From: Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation
Nav1.2 channels are necessary for proper elaboration of pre-OL structures and interaction with axons. a Immunostaining of CNPase-GFP+ cells for calretinin (red) and Nav1.2 (blue) in the MNTB (P21) following injection of empty vector (control, upper) and vector encoding shRNA1 for Nav1.2 (lower). Yellow arrows indicate shRNA virus-infected cells. Scale bar, 10 μm b Representative confocal images (upper) and 3D reconstruction (lower) of CNPase-GFP+ pre-OLs in control cells and in cells infected with scrambled shRNA, shRNA1, or shRNA2. Scale bar, 20 μm. c–e Summary of the data for cell volume c, total length of processes d, and soma diameter e under all conditions. All data represent the mean ± s.e.m.; *P < 0.05, **P < 0.01, ***P < 0.0001. n.s., nonsignificant difference
