Fig. 9
From: Oligodendroglial excitability mediated by glutamatergic inputs and Nav1.2 activation
Knockdown of Nav1.2 channels in pre-OLs impacts the myelination. a Confocal images of immunostaining for MBP in the auditory brainstem following the viral injection of vectors encoding scrambled shRNA (upper) and shRNA2 for Nav1.2 (lower). Scale bar, 20 μm. b Summary of the fluorescence intensity of MBP (red), which was measured in a 50 μm × 50 μm area surrounding CNPase-GFP+ cells within (white box). c Representative western blots probed for MBP and β-actin levels from three individual brainstem samples in each groups injected with scrambled shRNA (upper) and shRNA2. d Summary of normalized MBP levels. Detected western blot intensities for MBP were normalized to corresponding β-actin band intensities. All data represent the mean ± s.e.m.; *P < 0.05, **P < 0.01
