Fig. 4 | Nature Communications

Fig. 4

From: The catalytic subunit of DNA polymerase δ inhibits γTuRC activity and regulates Golgi-derived microtubules

Fig. 4

PolD1 depletion promotes Golgi-associated microtubule nucleation. A microtubule regrowth assay was performed on RPE1 cells transfected with control or PolD1 siRNAs. The cells were then stained for microtubules (anti-α-tubulin) and GM130. The images shown are representative of at least three experiments (100 cells analyzed per condition). Scale bar, 10 μm. Golgi-associated microtubules at 1 min of regrowth were counted and normalized by the Golgi mass. The data are presented as means ± s.d. and are representative of three independent experiments; ***p < 0.001, two-tailed, unpaired student’s t-test

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