Fig. 5
The miR-185-5p regulates expression of VEGF-A and VEGF-C. a Immunoblotting of HIF2α, VEGF-A, and VEGF-C with overexpressed or inhibited miR-185-5p in SW839 cells after 48 h treatment with miR-185-5p mimic or inhibitor. b Immunoblotting of HIF2α, VEGF-A, and VEGF-C in SW839 cells after 72 h treatment with miR-185-5p overexpressing plasmid or inhibitor. c Statistical results of a, b. d qPCR of HIF2α, VEGF-A, and VEGF-C after overexpressing miR-185-5p in SW839 cells for 48 h. e Immunoblotting of HIF2α, VEGF-A, and VEGF-C with/without knockdown of AR and overexpression of miR-185-5p in SW839 cells. f Statistical results of e. Triplicate experiments were carried out in all experiments. *and # p < 0.05, **p < 0.01 were considered statistically significant by t-test for two groups or ANOVA for more than two groups
