Fig. 2
From: Optical trapping of otoliths drives vestibular behaviours in larval zebrafish
Optical setup for otolith OT and behavioural imaging. Dorsal (a) and lateral (b) views, indicating the location of the utricular otoliths (circles) in a 6dpf zebrafish larva (scale bars, 200 µm). c Experimental set up (see Supplementary Methods for details) for delivering a dual OT to the larva using a 1064 nm fibre laser, a half-wave plate (HWP), polarising beam splitters (PBS), gimbal-mounted mirrors (GM), a dichroic mirror (DM), and lenses to project the two traps into the sample via a 20X 1NA microscope objective. Camera 1 (d) allows targeting of the OTs and imaging of the eyes (scale bar, 200 µm), and camera 2 (e) permits imaging of tail movements (scale bar, 600 µm)
