Fig. 5
From: Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90
Tumor-induced muscle wasting depends on tumor cell-expressed Hsp70 and Hsp90. a Conditioned medium of Hsp70- and Hsp90-deficient LLC cells do not induce myotube catabolism. C2C12 myotubes were treated with LCM from LLC cells transfected with random or Hsp70/Hsp90-specific siRNA as indicated. Catabolic response was evaluated as described in Fig. 2. Data (n = 3) were analyzed by analysis of variance (ANOVA). * denotes a difference (P < 0.05). b Serum Hsp70 and Hsp90 levels in mice bearing Hsp70- and Hsp90-deficient LLC tumor remain unchanged. LLC cells were transduced with lentivirus encoding Hsp70 and Hsp90-specific shRNA, or lentivirus carrying empty vector as control. Mice were implanted with Hsp70&90-deficient or control LLC cells. On day 21 serum levels of Hsp70 and Hsp90 were analyzed after Brij98 treatment. Data (n = 6) were analyzed by ANOVA. * denotes a difference (P < 0.05). c Hsp70&90-deficient LLC tumor does not induce muscle catabolism in mice. TA of mice derived from b was analyzed by western blotting for markers of catabolic pathways. Data (n = 6) were analyzed by ANOVA. * denotes a difference (P < 0.05). d Hsp70&90-deficient LLC tumor do not cause muscle wasting in mice. Mice derived from b were further analyzed for muscle wasting. Scale bar, 100 μm. Data (n = 6) were analyzed by ANOVA or χ 2 analysis (for CSA). * denotes a difference (P < 0.05)
