Fig. 7

Vesicular RSV filament formation is microtubule and dynein dependent. a Vero cells were infected with RSV A2 at an MOI of 1. At 12 hpi, SBA-488 was delivered to the cells and allowed to internalize for 1 h. Cells were then imaged live briefly at 9 Hz prior to drug delivery to verify the presence vesicular filament formation. Cells were then treated with nocodazole to disrupt microtubules, cytochalasin D to disrupt actin filaments, tetracaine to inhibit molecular motors, or EHNA to inhibit dynein before being imaged live again. Data is presented as SD maps over 30 s of imaging. Yellow arrows indicate fast motion, shown as streaks, while yellow arrowheads indicate slow motion, shown as hollow circles. Scale bars represent 5 µm. b Tubulin-GFP (green) was expressed in Vero cells overnight prior to infection with RSV A2 at an MOI of 1. At 12 hpi, SBA-647 (red) was delivered and allowed to internalize for 1 h. Cells were then imaged live at 4.9 Hz. Scale bar represents 3 µm