Fig. 8

RNP granules merge with glycoprotein vesicles prior to the plasma membrane. a Vero cells were infected with RSV at an MOI of 1. 12 hpi, SBA-488 (green) was delivered and allowed to internalize for 2 h. Cells were then fixed with 1% PFA, permeabilized with 0.1% saponin, and stained for RSV F (blue) and either RSV P, RSV N, or RSV M, as indicated (red). Scale bar represents 10 µm in whole-cell images and 1 µm in cropped images. Enlargement of cropped image from the white boxed region with an intensity profile showing colocalization along the direction of the white arrow. b The same experiment in a was repeated in SBA-488 (green) stained HEp-2 cells. Cells were stained for RSV F (blue) and either RSV P, RSV N, or RSV M as indicated (red). Scale bar represents 10 µm in whole-cell images and 1 µm in cropped images. Enlargement of cropped image from the white boxed region with an intensity profile showing colocalization along the direction of the white arrow