Fig. 5
From: Foxp3+ Tregs are recruited to the retina to repair pathological angiogenesis
Expanding Treg numbers altered the activation state of microglia. Flow cytometry of retina from C57BL/6J mice at postnatal day 10 in phase I OIR. Fluorochromes are AF700, AF488 and phycoerythrin (PE). a FACS plot schema showing cell populations in OIR retina revealed by CD45 and CD11b staining. Microglia (CD45midCD11b+) rather than macrophages (CD45highCD11b+) predominate and Tmem119 is restricted to CD45midCD11b+ microglia. b Mean fluorescence intensity (MFI) of CD86 in CD45midCD11b+Tmem119+ microglia showing an increase with OIR compared to room air controls (RA), and a reduction in OIR mice following treatment with the IL-2/anti-IL-2 mAb complex and adoptively transferred (AT) Tregs. c MFI of CD86 in CD45highCD11b+Tmem119− macrophages showing no change with OIR compared to RA or the treatments. **P < 0.01 to RA. ## P < 0.01 to OIR control (one-way ANOVA with Mann–Whitney U test). NS, not significant. n = 5 mice group. Values are expressed as mean ± s.e.m
