Fig. 6

Expanding Treg numbers preserved the ramified phenotype of microglia. Representative confocal images of flat mounted retina from C57BL/6J mice showing blood vessels (isolectin, green), microglia (Iba1⁺, red), and nuclei (DAPI, blue). Scale bar, 100 μm. In room air controls (RA) at a P12 and b P18, Iba1⁺ cells with long ramified processes (arrows) predominate, while in OIR at both time points, most Iba1⁺ cells exhibit short cell processes (asterisks) and are found in the vaso-obliterated (VO) retina. In OIR mice treated with the IL-2/anti-IL-2 mAb complex or the adoptive transfer (AT) of Tregs, the number of Iba1⁺ cells with long ramified cell processes (arrows) predominate. The primary cell process of each Iba1⁺ cell per field of retina was quantitated at c P12 and d P18. *P < 0.05 to RA. ^# P < 0.05 to OIR control (one-way ANOVA with Mann–Whitney U test). n = 6 room air control mice and 8 mice/OIR group. Values are expressed as mean ± s.e.m