Fig. 4
From: Combined activation of MAP kinase pathway and β-catenin signaling cause deep penetrating nevi
Mutant CTNNB1 confers increased cell volume, pigmentation and cyclin D1 levels in vitro. a Stably transduced melanocytes (melan-a) with BRAFV600E and CTNNB1S33F demonstrate increased β-catenin activity as measured by TOP flash luciferase expression (left; P = 0.03), cell volume (center; P = 0.04) and melanin content (right; P = 0.007) as compared to melanocytes transduced with BRAFV600E alone. Mean with error bars showing s.e.m., unpaired Student’s t-test, three biological replicates performed. b Representative western blot analysis of melanocytes stably transduced with BRAFV600E and CTNNB1S33F demonstrate increased cyclin D1 levels as compared to those tranduced with GFP or BRAFV600E. Data shown are representative of at least three independent experiments with similar results
