Fig. 1
From: Slow-wave sleep is controlled by a subset of nucleus accumbens core neurons in mice
Chemogenetic stimulation of A2AR neurons in the NAc induced SWS. a A2AR-Cre mice were injected with AAV-hSyn-DIO-hM3Dq-mCherry into the NAc and implanted with somnographic electrodes. b A brain section was stained against mCherry to confirm that hM3Dq-mCherry protein was expressed in the NAc. Scale bar: 500 μm. c Time course of locomotor activity. Black and white bars indicate dark and light periods, respectively. Two-way repeated measures ANOVA was performed followed by Bonferroni’s post hoc comparisons. *P < 0.05, **P < 0.01, compared with vehicle. d Typical examples of EEG (power spectrogram and wave traces), EMG, and hypnograms after the administration of vehicle or CNO. e Dose-dependent changes in SWS time normalised to the SWS time of the vehicle control. One-way ANOVA was performed followed by Bonferroni’s post hoc comparisons. f SWS episode spectrum. *P < 0.05, compared with vehicle, assessed by paired two-tailed Student’s t-test. g EEG power density of SWS between 20:00 and 23:00. Data are presented as the mean ± SEM (n = 6). Each pair of grey dots indicates data from one mouse
