Fig. 5
From: Slow-wave sleep is controlled by a subset of nucleus accumbens core neurons in mice
Chemogenetic inhibition of A2AR neurons in the NAc core reduced sleep. a A2AR-Cre mice were injected with AAV-hSyn-DIO-hM4Di-mCherry into the NAc core. b Brain sections were stained against mCherry to confirm that hM4Di-mCherry protein was expressed in the NAc core. Scale bar: 500 μm. c Time course of SWS. d CNO decreased dose-dependently the total amount of SWS. e, f CNO decreased number of SWS episodes e without affecting the mean duration f. g SWS amount for 7 h after sleep deprivation in the absence and presence of CNO. h, i SWS rebound after sleep deprivation h and CNO-induced SWS reduction i were not affected by CNO administration and sleep deprivation, respectively. Data are presented as the mean ± SEM (n = 6). Each pair of grey dots indicates data from one mouse. *P < 0.05, **P < 0.01 compared with vehicle, assessed by two-way c, g, one-way d–f repeated measures ANOVA followed by Bonferroni’s post hoc comparisons, or paired two-tailed Student’s t-test h, i. N.S., not significant
