Fig. 2

CFTR^−/− swine fail to respond to Pseudomonas aeruginosa in vivo. Scatter plot and median (red line) of the effect of a bacteria-free and b bacteria-laden agarose beads on the ASL layer of wild-type swine. Bacteria-free beads trigger an ASL increase from 105 to 123 µm (P < 0.05, n = 10 from seven pigs, Wilcoxon match-pairs signed rank test) while bacteria-laden beads triggered a height increase from 117 to 168 µm (P < 0.05, n = 10 from six pigs, Wilcoxon match-pairs signed rank test). c Increase in ASL height triggered by bacteria-laden (n = 10) and bacteria-free (n = 10) agarose beads (P < 0.0001, t = 7.76, df = 18, Student’s t-test). Effect of d bacteria-free and e bacteria-laden agarose beads on the ASL layer of CFTR^−/− swine. Bacteria-free beads trigger an ASL increase from 172 to 178 µm (P < 0.05, n = 9 from six pigs, Wilcoxon match-pairs signed rank test) while bacteria-laden beads triggered a height increase from 150 to 158 µm (P < 0.05, n = 11 from eight pigs, Wilcoxon match-pairs signed rank test). f The increase in ASL height triggered by bacteria-laden (n = 11) and bacteria-free (n = 9) agarose beads were not significantly different in CFTR^−/− swine (P = 0.49, Mann–Whitney test). Data are presented as mean ± SEM and within each panel the columns labeled with an asterisk differ significantly