Fig. 2

Retrotransposon LINE1 derepression in Pnldc1 mutant spermatocytes. a In situ hybridization of LINE1 Orf1 mRNA in adult WT and Pnldc1 ^Mut (Mut-1) testes. LINE1 Orf1 mRNA was upregulated in spermatocytes in Pnldc1 ^Mut testes but was undetectable in WT testes. Scale bar, 50 μm. b Immunostaining was performed using LINE1 ORF1 antibody on adult testis sections from WT and Pnldc1 ^Mut (Mut-1) mice. LINE1 ORF1 was upregulated in spermatocytes in Pnldc1 ^Mut testes but was undetectable in WT testes. Scale bar, 20 μm. c Immunostaining was performed using LINE1 ORF1 antibody and γH2AX antibody on adult testis sections from WT and Pnldc1 ^Mut (Mut-1) mice. Different cell types were distinguished according to γH2AX staining and DAPI staining. LINE1 ORF1 was expressed from zygotene spermatocytes to mid-pachytene spermatocytes in Pnldc1 ^Mut testes. Scale bar, 5 μm