Fig. 3 | Nature Communications

Fig. 3

From: PNLDC1 is essential for piRNA 3′ end trimming and transposon silencing during spermatogenesis in mice

Fig. 3

Pnldc1 mutation causes mitochondria disorder. a Expression of TDRKH, MILI, MIWI, GASZ, and MVH in WT and Pnldc1 Mut (Mut-1) testes revealed by western blotting. β-actin is a loading control. b Aggregation of TDRKH, MILI, MIWI, and GASZ in Pnldc1 Mut spermatocytes. Immunostaining was performed using indicated antibodies on adult testis sections from WT and Pnldc1 Mut (Mut-1) mice. DNA (blue) is stained with DAPI. Protein aggregations are indicated by arrows. Scale bar, 10 μm. c Conglomeration of mitochondria in Pnldc1 Mut testes. Immunostaining of WT and Pnldc1 Mut (Mut-1) testis sections with an antibody against AIF, a mitochondrial marker. DNA (blue) is stained with DAPI. Conglomeration of mitochondria is indicated by arrows. Scale bar, 10 μm

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