Fig. 7
PNLDC1 is required for pre-pachytene piRNA trimming. a Immunostaining of MILI on newborn (P0) testis sections from Pnldc1 HET and Pnldc1 KO mice. Scale bar, 10 μm. b Immunostaining of MIWI2 on testis sections from Pnldc1 HET and Pnldc1 KO mice. MIWI2 is localized in both cytoplasm and nucleus in P0 Pnldc1 KO testes. Scale bar, 10 μm. c Immunostaining of MIWI2 on P0 testis sections from Tdrkh HET and Tdrkh KO mice. MIWI2 exclusively localizes in the cytoplasm of P0 Tdrkh KO germ cells. Scale bar, 10 μm. d The length distribution of MILI-piRNAs from P0 Pnldc1 HET and Pnldc1 KO MILI-piRNA libraries. e piRNA 3′ end extension in P0 Pnldc1 KO mice. Alignment of 31–40 nt reads from P0 Pnldc1 KO MILI-piRNA library and 24–30 nt reads from P0 Pnldc1 HET MILI-piRNA library within a selected region in one representative piRNA cluster. The genomic location of this piRNA cluster is shown at the bottom
