Fig. 2
From: Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
Complete assessment of the efficiencies and specificities of TALE RVDs towards 5mC and 5hmC. a A heat map summarizing the screening data of 5mC and 5hmC. For easy comparison, the results for unmodified C and T reporters are also shown. EGFP activities from different reporters are coded using different colors representing the reporter identities (5mC, green; 5hmC, yellow; T, red; C, blue), and the brightness of the colors indicates the fold induction of reporters by TALE-(XX′)3 normalized to the basal levels. The single-letter abbreviations for the amino acids are used. The upper right side legend shows the specific heat map of Y*. Starting from the top left, the DNA reporter is 5mC, 5hmC, T and C, respectively. b Modified cytosine preference of RVDs. RVDs are clustered according to base preference and are categorized by the 13th residue in each cluster. The data are means + s.d., n = 3; *P < 0.05, and **P < 0.005 two-tailed unpaired Student’s t test
