Fig. 4
From: Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
Methylation-dependent gene expression activation and genome editing. a TALETET1 targets a 16 bp DNA sequence ~80 bp upstream of the transcription start site (TSS) of the TET1 gene. All three CpGs (whose Cs are indicated in red) in the region are highly methylated in HeLa cells but unmethylated in HEK293T cells. b The relative mRNA level of TET1 in HeLa and HEK293T cells transfected with TALETET1 containing different RVDs. c TALELRP2 targets a 16 bp sequence ~ 100 bp upstream the TSS of the LRP2 gene. Both the two CpGs in this region contain medium-level methylation in HeLa cells while are unmethylated in HEK293T cells. d The relative mRNA level of LRP2 in HeLa and HEK293T cells transfected with TALELRP2 containing different RVDs. e The position of TALEN targeted sequence. The methylated CpGs are indicated in red. f The genome editing efficiency of TALEN using different RVDs. The data are means + s.d., n = 3; *P < 0.05, and **P < 0.005 two-tailed unpaired Student’s t test
