Fig. 5
Infusion of PDBu in PVA induce cardioprotection in naïve animals. a Schematic of experimental design showing the timeline for cannulation (D0), intra-PVA infusion of PDBu (20 pmol in 0.3 μL 50% dimethyl sulfoxide (DMSO)) or 50% DMSO (D1, D2), and myocardial IR surgery (D3). b Mechanical responses of hind paws at D0 (basal), D1, D1 4 h after first PDBu infusion, D2, D2 4 h after 2nd PDBu infusion and D3. *p < 0.05 vs. DMSO group. Arrow indicates intra-PVA infusion of PDBu. c Immunohistochemical staining of pERK in PVA (outlined by dashed line) from animals received PDBu and DMSO infusion. Scale bar = 100 μm. PDBu*1, infusion of PDBu (D1) and infusion of 50% DMSO (D2) in PVA. PDBu*2, infusion of PDBu at D1 and D2 in PVA. PDBu off-site, infusion of PDBu at D1 and D2 in CA1 region of hippocampus. Scale bar = 100 μm. d Representative images of TTC staining in heart cross-sections. Quantification results of infarct size and AAR in cardiac sections from different treatment groups (lower panel). Two infusions of PDBu in PVA were sufficient to induce cardioprotection in naïve animals. *p < 0.05 vs. DMSO group. Error bars indicate SEM. Statistical significance was determined by two-way RM ANOVA (b) or one-way ANOVA (d)
