Fig. 7
Inhibition of PVA neuron with DREADD alleviates SNI-induced cardioprotection. a Immunofluorescent images of AAV-hM4D-mCherry (red)-positive signals in PVA regions (scale bar = 100 μm). Evoked spontaneous action potentials recorded from hM4D-mCherry-positive PVA neurons with CNO bath perfusion. b Schematic of experimental design showing the timeline for SNI (D0), CNO IP injection (D5) and IR injury (3 h after CNO injection). c Representative images of TTC staining in heart cross-sections. Quantification results of infarct size and AAR in cardiac sections from eYFP and hM4D groups in sham and SNI animals. Activation of hM4D with CNO alleviates SNI-induced cardioprotection. *p < 0.05 vs. eYFP group. Error bars indicate SEM. Statistical significance was determined by one-way ANOVA (c)
