Fig. 8
Parasympathetic but not sympathetic blockers prevent SNI-induced and PVA PDBu infusion-induced cardioprotection. a Schematic of experimental design showing the timeline for SNI surgery (D0), i.v. infusion of blockers (D5), and myocardial IR surgery (D5) (left panel). Right panel shows the timeline for PVA PDBu infusion (D1, D2), i.v. infusion of blockers (D3), and myocardial IR surgery (D3). b Representative images of TTC staining in heart cross-sections from different treatment groups (left panels). Quantification results of infarct size and AAR in cardiac sections different groups (right panels). Hexamethonium (30 mg/kg), propranolol (2 mg/kg), glycopyrrolate (0.4 mg/kg) were infused i.v. 15 min prior to myocardial ischemia surgery in mice received sham or SNI surgery. *p < 0.05 vs. sham group. Hexamethonium and glycopyrrolate but not propranolol prevented SNI-induced cardioprotection. c Representative images of TTC staining in heart cross-sections in mice treated with vehicle or glycopyrrolate (left panels). Quantification results of infarct size in cardiac sections (right panels). *p < 0.05 vs. vehicle group. Error bars indicate SEM. Statistical significance was determined by Student’s t-test (b, c)
