Fig. 5

Impact of vrs3 on grain size and uniformity in the six-rowed spike. a A typical six-rowed barley spikelet triplet (vrs1.a, Int-c.a, Vrs3) showing the central grain (Cen) flanked by two lateral grain (Lat). Scale bar, 1 cm. b, c Six-rowed barley spikes from b the current commercial six-rowed genotype model: vrs1.a, Int-c.a, Vrs3.w (662) and c with the introduction of vrs3: vrs1.a, Int-c.a, vrs3 (666), respectively; Scale bar, 1 cm. d, e Distributions of d central and e lateral grain width fractions within the 662 genotypes. f, g Distributions of f central and g lateral grain width fractions within the 666 genotypes. h–j comparison of the mean grain width, grain area and lateral to central grain area ratio between the 662 (n = 6) and 666 (n = 13) genotype combinations; black bars: 662 genotype, grey bars: 666 genotype. Error bars are ± S.E.D