Fig. 6
From: An NF-κB-microRNA regulatory network tunes macrophage inflammatory responses
Mir-155 acts as an amplifier in suboptimal stimulation. WT, miR-155−/−, miR-146a−/− and double knock out (DKO) BMMs were stimulated with low levels of LPS (10 ng/mL) (a) or Pam3CSK4 (5 ng/mL) (b). mRNA levels of IL-1β (left) and IL-6 (right) were then quantified using qRT-PCR. c TNF and phospho-p65 (P-p65) protein expression of BMMs from WT (grey), miR-155−/− (blue), miR-146a−/− (red) and dKO (green) 4 h after stimulation with low levels of LPS (10 ng/mL) as measured by FACS using intracellular staining. N = 4 per group from at least two independent experiments, represented as mean ± SEM. a, b *p < 0.05, using two-way ANOVA
