Fig. 2

The SCW is mediated by the RhoA/Rok/NMYII module. a Selected frames of a time-lapse recording of an oocyte expressing the heavy chain of non-muscle myosin II (NMYIIhc)-mEGFP during SCW. Single confocal slice along the equatorial plane across the A–V axis; scale bar 20 μm; time is in mm:ss. For the complete movie see Supplementary Movie 2. b Kymographs showing radii of curvature as in Fig. 1c, and the fluorescence signal of NMYIIhc-EGFP measured at the cortex and in a subcortical region simultaneously over time, as illustrated by the scheme on the left. Underneath, fluorescence intensity plot of the cortical (solid line) and subcortical NMYIIhc-EGFP (dashed line) signal, compared to curvature change (gray line) averaged along the wave front. c Scheme illustrating the signaling pathway controlling SCWs in starfish oocytes and the inhibitors used in this study to interfere with the respective components. d–f Quantification of the strength of the shape change during the SCW for oocytes treated with inhibitors as indicated. Dot plots of measurements of individual oocytes overlaid with box plots of the same data. ***p < 0.001, **p < 0.01, n.s. not significant, determined via ANOVA. g Selected frames of a time-lapse recording of an oocyte expressing RhoA-GTP reporter (EGFP-rGBD) during the SCW and corresponding kymographs of curvature radii and cortical fluorescence intensities as in b. Single confocal slice along the equatorial plane across the A–V axis is shown; scale bar=20 μm, time is in mm:ss. For the complete movie see Supplementary Movie 3. h Oocyte expressing EGFP-rGBD and imaged as in g, injected with the Rok inhibitor Y-27632, selected frames and kymographs of radii of curvature and cortical fluorescence intensities are shown. Scale bar=20 μm; time is in mm:ss. For the complete movie see Supplementary Movie 4