Fig. 3
From: A cdk1 gradient guides surface contraction waves in oocytes
cdk1-cyclinB forms a spatiotemporal gradient controlling the RhoA module. a Oocyte expressing RhoA-GTP marker EGFP-rGBD and locally treated with DMSO (as in scheme to the left), starting point of SCW marked by a red asterisk. Right: Kymograph of the cortical rGBD signal. Scale bar=20 μm. b Same as a except oocyte was globally treated with the cdk1 inhibitor RO-3306. c Same as a except oocyte was locally treated with the cdk1 inhibitor RO-3306, the starting point of the SCW is marked by a red asterisk. d Pseudo-colored frames from a time-lapse recording of an oocyte expressing cyclinB-EGFP during meiosis I. Right: contrast adjusted to visualize decreased levels of cyclinB during SCW. Images are 15 s averages of 1.5 s per frame recording. Scale bar=20 μm; time relative to NEBD, in mm:ss. See also Supplementary Movie 5. e Quantification of cyclinB-mEGFP intensities of the oocyte shown in d. Left: kymograph of the subcortical cyclinB-EGFP fluorescence intensity during meiosis with intensity-isolines in white. Middle: Zoom of the white dashed box including the time of the SCW with adjusted contrast and the isoline conforming to the SCW highlighted in red. Right: kymograph of radii of curvature during the same time window with the isoline from the middle plot overlaid. f Kymograph of the simulated cdk1-cyclinB concentration profile along the cortex. For details of the simulation see Methods. g The reaction system of cdk1-cyclinB inactivation. h Frame of the 3D finite element simulation of the cdk1-cyclinB reaction-diffusion system. i For the oocyte shown in d, cyclinB-EGFP subcortical intensities plotted over time at angles from animal pole to vegetal pole (darkest to lightest gray), smoothed data shown in bold, raw data in thin line. Red line same as isoline in e. j For the oocyte shown in d, subcortical cyclinB-EGFP intensities plotted from the animal to vegetal pole during the SCW at time points 40, 43, 46, 49, 52, and 55 min after NEBD (darkest to lightest gray). Red line same as isoline in e. k Simulated cdk1-cyclinB intensity as in i. l Simulated cdk1-cyclinB intensity profiles as in j
