Fig. 2
PI3Kγ or PI3Kδ inhibition suppresses acute rejection. Naive BALB/c heart allografts were transplanted into fully allogeneic C57BL/6 (WT), PI3Kγ −/−, or PI3Kδ D910A/D910A recipients. a PI3Kγ −/− recipients exhibited prolonged allograft survival compared to WT recipients (MST of 11 vs. 7 days respectively, *p < 0.05, t-test, n = 10/group). PI3Kδ D910A/D910A recipients of BALB/c hearts showed significant prolongation of heart allograft survival compared to control (MST of 14 vs. 7 days, respectively, *p < 0.05, t-test, n = 10–14/group). b Representative examples of H&E staining of cardiac allograft histology show well-preserved myocytes and lower cellular inflammatory infiltrate at 7 days post-transplant in the PI3Kγ –/– mice compared to WT controls (Scale bar 50 μm, inset scale bar 25 μm). c Representative examples of cardiac allograft histology show well preserved myocytes and lower cellular inflammatory infiltrate at 7 days post-transplant in the PI3Kδ D910A/D910A mice compared to WT controls (H&E stain, scale bar 50 μm, inset scale bar 25 μm). d Representative dot plots of Tregs (CD4+FoxP3+) in splenocytes of WT and PI3Kδ D910A/D910A recipients at day 7 post-transplant. Bar graph represents the percentage of Tregs in splenocytes in PI3Kγ −/− recipients compared to WT at day 7 post-transplant (*p < 0.05, t-test, n = 6/group, the graph shows data as mean ± s.e.m.). (MST mean survival time, Treg regulatory T cell)
