Fig. 3
NatD promotes lung cancer cell invasion in a xenograft mouse model. a (left) Representative bioluminescent (BLI) images acquired at the indicated time points after intravenous injection of SCID mice with Scr or NatD-KD A549 cells. NatD-KD2 cells were used because the KD2 shRNA produced a better knockdown effect. Pseudocolor heat-maps indicate intensity of bioluminescence from low (blue) to high (red) (D, day). (right) Normalized BLI signals of lung tumors of corresponding mice (n = 5 for each group) recorded at the indicated time points. Results are shown as mean ± s.d. from five mice. Two-tailed Student’s t-test was used. *P < 0.05 compared to Scr control. b (left) Representative images of lung nodules of SCID mice acquired 28 days after intravenous injection with Scr or NatD-KD A549 cells. (middle) Representative images of H&E stained histological sections of lungs from SCID mice. Scale bars, 2 mm. Arrows indicate major metastatic nodules. (right) Box plot showing numbers of lung nodules from corresponding mice (n = 5 for each group). Results are shown as mean ± s.d. from five mice. Two-tailed Student’s t-test was used. *P < 0.05 compared to Scr control. c (left) Representative images showing luciferase activity in lungs from SCID mice as in (b). (right) Quantification of total lung bioluminescence from SCID mice as in (b) (n = 5 for each group). Results are shown as mean ± s.d. from five mice. Two-tailed Student’s t-test was used. *P < 0.05 compared to Scr control
