Fig. 3
A complete CME event of a gold nanorod by an A549 cell. Corresponding video are presented in Supplementary Movies 1–6. Time 0 is set to the moment when the nanorod first appeared on the membrane. a The cell and the location of the gold nanorod probe on the cell membrane. The nanorod (on the left in the red box) was beside a cell feature (on the right in the red box) that showed a constant contrast throughout the recording. b The trace of the nanorod’s active translational movement at an early binding stage (Supplementary Movie 2). c DIC intensities of the nanorod in the same period as b. The framed period shows typical out-of-plane rotations. d DIC intensities of the nanorod at a late stage (Supplementary Movie 3). The framed period shows typical in-plane rotations. e Sequential DIC and fluorescence images captured in tandem showing that the gold nanorod co-localized with a CCP (Supplementary Movie 4). The movie was acquired at 32 frames per second continuously and the displayed fluorescence images were the average of 10 consecutive images from the movie. f DIC intensities of the nanorod before and after scission of the nanorod-containing vesicle (Supplementary Movie 5). The blank period from 531.5 to 532.8 s was toggled to fluorescence mode in checking clathrin fluorescence. The nanorod lost active rotation at 536.5 s and regained rotational and translational freedoms at 553.7 s. The same particle was observed to lose co-localization of fluorescence from EYFP-clathrin, indicating the clathrin disassembly (Supplementary Movie 6). Supplementary Movie 7 shows that the particle was actively transported at 45.7 s after scission in DIC mode
