Table 1 Parameters of endothelin 1 and chymotrypsin captured with WtFraC and ReFraC

From: Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores

 

WtFraC

 

ReFraC

 

pH 7.5

pH 4.5

 

pH 7.5

pH 4.5

 

τ on (ms)

τ off (ms)

τ on (ms)

τ off (ms)

 

τ on (ms)

τ off (ms)

τ on (ms)

τ off (ms)

Endothelin 1 (−50 mV)

–

–

5.8 ± 0.7

5.6 ± 2.0

Endothelin 1 (+50 mV)

1413.7 ± 286.9

3.3 ± 2.2

401.7 ± 79.4

8.5 ± 1.8

Chymotrypsin (−100 mV)

4.4 ± 1.9

12.0 ± 5.7

9.6 ± 2.5

1.7 ± 0.9

Chymotrypsin (+200 mV)

174.3 ± 22.9

0.2 ± 0.1

112.5 ± 9.5

1.3 ± 0.7

  1. The buffer at pH 7.5 consisted of 1 M KCl, 15 mM Tris base. The buffer at pH 4.5 consisted of 1 M KCl, 0.1 M citric acid, 180 mM Tris base. Endothelin 1 and chymotrypsin were added into cis compartment. Recordings were performed using a 50 kHz sampling rate with a 10 kHz low-pass Bessel filter. The errors represent the standard deviations calculated from three experiments
  2. τ on inter-event time, τ off dwell time
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