Fig. 1

Intact CD23⁺ B-cell compartment despite deletion of Prmt1. a Representative flow cytometry plots examining the frequency of mature (CD19⁺CD93⁻), immature (CD19⁺CD93⁺), follicular (CD19⁺CD93⁻CD23⁺CD21⁺) and MZ (CD19⁺CD93⁻CD23⁻CD21⁺) B cells in the spleen of control (+/+) and Prmt1 ^f/fCD23Cre (f/f) mice. Numbers indicate percentage of the displayed events. b, c Number of splenocytes and B cells are indicated. d Representative immunohistochemical staining of frozen sections from spleens of control (+/+) and Prmt1 ^f/f CD23Cre (f/f) naive mice with scale bars indicating 100 μm. All data are representative of three independent experiments and n = 10 mice. Statistical significances were determined using Student’s t-test; ns = not significant. Mean and s.e.m. in b and c. Flow cytometry gating strategies for this figure are shown in Supplementary Fig. 6