Fig. 4
From: Mechanism of error-free replication across benzo[a]pyrene stereoisomers by Rev1 DNA polymerase
The 10R (+)-cis-BP-N 2-dG–modified Rev1 ternary complex. a Overall structure of the complex. The coloring scheme and the details shown are as in Fig. 2. The template 10R (+)-cis-BP-N 2-dG is in beige. b Alignment of the 10R (+)-cis-BP-N 2-dG adduct in the Rev1 active site. The 10S (+)-trans-BP moiety is positioned in the solvent-filled space between the PAD and the template–primer DNA helix. c A simulated annealing Fo−Fc omit map (contoured at 3.5σ at 1.92 Å resolution and colored in blue) showing clear density for the BP moiety and the modified dG. The dG as well as the C9-OH and C8-OH hydroxyl groups are oriented below the BP benzylic ring, while the C7-OH is above the ring. This confirms the expected stereochemistry of the 10R (+)-cis BP benzylic ring
