Fig. 4
In vivo and ex vivo imaging with H-MnO2-PEG/C&D. a In vivo fluorescence images of 4T1 tumor-bearing mice taken at different time points post i.v. injection of H-MnO2-PEG/C&D (three mice per group). b Ex vivo fluorescence images of major organs and tumor dissected from mice at 24 h p.i. Sp, Ki, H, Lu, In, Li, and Tu stood for spleen, kidney, heart, lung, intestine, liver, and tumor, respectively. c Semi-quantitative analysis of ex vivo fluorescence images in different organs in (b). d T1-weighted MR images of the H-MnO2-PEG/C&D recorded using 3T MR scanner at different pH values (6 and 7.4). The transverse relativities (r1) were 8.743 and 0.051 mM−1 s−1 for H-MnO2-PEG/C&D at pH 6 and 7.4, respectively. e T1-MR images of 4T1 tumor-bearing mice before and after local injection of H-MnO2-PEG/C&D within normal and tumor subcutaneous tissues (three mice per group). f Quantified T1-MR signals in muscle and tumor before and after injection of H-MnO2-PEG/C&D based on images in (e). g, h In vivo T1-MR images of a mouse taken before and 24 h post i.v. injection of H-MnO2-PEG/C&D shown in cross section (g) and longitudinal section (h). i Quantification analysis of T1-MR signals in liver, kidney, and tumor, before and 24 h post i.v. injection or H-MnO2-PEG/C&D nanoparticles. Date are presented as means ± s.d. (n = 3 mice per group)
