Fig. 2

Both TRPA1 and TRPV1 channels are involved in SADBE-induced persistent itch. a Schematic protocol of nociceptor ablation and induction of SADBE-induced ACD model; (b) Schematic protocol of SADBE-induced ACD model; (c) SADBE-induced spontaneous scratching in wt mice pre-treated with vehicle or RTX. Data are presented as mean ± SEM. Asterisks indicate statistical significance. **p < 0.01, Student’s t-test; (d) SADBE-induced spontaneous scratching in wt, Trpa1 ^−/−, Trpv1 ^−/−, and Trpa1 ^−/−/Trpv1 ^−/− dKO mice. Data are presented as mean ± SEM. Asterisks indicate statistical significance. *p < 0.05; **p < 0.01; ****p < 0.0001, ANOVA