Fig. 4
From: Sensory TRP channels contribute differentially to skin inflammation and persistent itch
Structural basis of SADBE activation of recombinant TRPA1 and TRPV1. a, b Representative time-lapse traces illustrate SADBE-elicited [Ca2+]i responses in HEK293 cells transfected with TRPA1 (a) and TRPV1 (b) constructs, which could be also activated by AITC and capsaicin, respectively; c, d Representative current traces illustrate that SADBE activated large outward (at +100 mV) and inward currents (at −100 mV) in HEK293 cells transfected with TRPA1 (c) and TRPV1 (d) constructs, which were also activated by AITC or capsaicin, respectively; e, f Representative I-V curves illustrate that SADBE-activated outwardly rectifying TRPA1 (e) and TRPV1 (f) currents. Note that the TRPA1-expressing HEK293 cell was activated by AITC and the TRPV1-expressing cell was activated by capsaicin; g Concentration–response relationships of SADBE in wild-type and mutant TRPA1 channels; h Concentration–response relationships of SADBE in wild-type and mutant TRPV1 channels
