Fig. 5

TRPA1 is the primary mediator of SADBE-induced acute itch. a Bar charts illustrate acute itch and pain behaviors in wt mice by cheek injections of SADBE (30 mM). Facial wiping with the forelimb was indicative of a pain response, whereas scratching with the hind paw was considered as an itch response. Asterisks indicate statistical significance. Data are presented as mean ± SEM. NS, not significant. **p < 0.01, Student’s t-test; b Bar charts illustrate acute itch responses elicited by intradermal injections of vehicle or SADBE (30 mM) in the neck of wt, Trpa1 ^−/−, Trpv1 ^−/− and Trpa1 ^−/−/Trpv1 ^−/− dKO mice. Data are presented as mean ± SEM. NS, not significant. Asterisks indicate statistical significance. *p < 0.05; **p < 0.01, ANOVA