Table 1 Crystallographic data collection and refinement statistics
ΔIBB-importin α3:RCC1 | IBB-Kap60: yRCC1 | |
|---|---|---|
Data collection | ||
Space group | P1 | P212121 |
Cell dimensions | ||
a, b, c (Å) | 127.4, 162.5, 161.6 | 91.1, 99.2, 125.0 |
α, β, γ (°) | 75.7, 85.6, 72.2 | 90.0, 90.0, 90.0 |
Wavelength (Å) | 0.97 | 1.18 |
Resolution (Å) | 50–3.45 (3.53–3.45) | 50–2.63 (2.72–2.63) |
No. of reflections (tot/unique) | 935,054/143,482 | 160,524/33,934 |
R sym | 13.1 (65.5) | 4.9 (64.4) |
R pim | 11.2 (65.9) | 2.2 (42.8) |
I/σI | 12.2 (1.8) | 49.9 (3.4) |
Completeness (%) | 90.4 (71.5) | 96.0 (85.8) |
Redundancy | 2.0 (1.6) | 4.7 (2.7) |
Refinement | ||
PDB ID | 5TBK | 5T94 |
Resolution (Å) | 20–3.45 | 15–2.63 |
No. of reflections | 118,199 | 31,489 |
R work/R free* | 27.8/29.6 | 21.7/25.9 |
No. of complexes in AU | 8 | 1 |
No. of protein atoms | 50,677 | 6808 |
Ramachandran | ||
Favored/allowed/outliers | 96.1/3.9/0.0 | 95.3/4.6/0.1 |
MolProbity Clashscore | 8.8 | 11.6 |
R.m.s deviations | ||
Bond lengths (Å) | 0.005 | 0.002 |
Bond angles (°) | 0.684 | 0.496 |
