Fig. 6
From: Mutant p53 shapes the enhancer landscape of cancer cells in response to chronic immune signaling
Mutp53 enhancer and gene activation in human colon tissues. a qRT-PCR analysis of MMP9 eRNA from the −3.7 kb enhancer and mRNA expression levels from five independent cases of paired non-neoplastic (NT) and colorectal carcinomas (CRC) expressing wild-type and mutant forms of p53, respectively. mRNA expression levels of NT and CRC samples were normalized to β-actin levels. The expression levels shown for each CRC sample is relative to the expression levels of its corresponding NT sample. b Proposed model in which mutp53 through interactions with NFκB directs enhanced RNAPII recruitment that is required for the potent induction of enhancer transcription and pro-tumorigenic gene expression in response to chronic TNF signaling. The contributions of wild-type p53 were also examined in this study and are included in our model to show that the TNF-α-induced changes in gene expression are mutp53-dependent as wild-type p53 is neither recruited to the mutp53-bound enhancer regions, nor regulates enhancer activation and gene induction
