Fig. 3
From: Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq
Sfpq, Pspc1, and NonO interact with Ago2 in the nucleoplasm. a Immunoblot analysis of chromatin, nucleoplasm, or cytoplasm from HeLa, HEK293T, P19, or RA-treated P19 cells using antibodies directed to the indicated proteins. b Co-immunofluorescence analysis of RAW 264.7 cells stained with the indicated antibodies. Scale bar corresponds to 10 μm. c Co-IP of endogenous Ago2 and the indicated proteins from nucleoplasmic or cytoplasmic extracts from RAW 264.7 or P19 cells
